Bacterial, Fungal, & Inclusion Body Stains
A variety of stains are available for determining the presence of fungi. Among the fungal stains, selectivity includes fungal mycelia and conidia, mucin, Rickettsia, and nuclei. Typical fixatives include buffered neutral formalin or Zenker’s Fixative. The most common fungal staining solutions are Coleman’s Feulgen with Metanil Yellow, Light Green Solution, Weigert’s Iron Hematoxylin, and Giemsa Solution with Lugol’s or Gram’s Iodine.
Inclusion Body Stains are selective toward inclusion bodies, erythrocytes, plasma, collagen, squamous epithelium, connective or elastic tissue, muscle, keratin, and nuclei. Buffered Neutral Formalin and Zenker’s are the preferred fixatives. Common staining reagents include Mayer’s Hematoxylin, Scott’s Water Solution, Lugol’s or Gram’s Iodine, and Harris Hematoxylin.
Blood Stains & Stains for Hematologic Elements
While no fixatives are often required, buffered neutral formalin is the most common fixative for blood stains. Zenker’s or methanol are other choices.
Popular reagents for blood and hematologic element staining include Wright Staining Solution, Giemsa Thin or Thick Film Staining Solution, Phloxine B, Hemalum, Gram’s or Lugol’s Iodine, Fouchet’s Reagent, Van Gieson’s Solution, Nuclear Fast Red (Kernechtrot Solution), and Mayer’s Hematoxylin.