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Hematoxylin is a natural product extracted from the heartwood of Hematoxylon campechianum trees using hot water and precipitated from solution using urea. When hematoxylin is oxidized, it yields the dye hematein. Oxidation can take place naturally via “ripening” in air (such as in the case of Ehrlich’s Hematoxylin or Delafield’s Hematoxylin solutions) or, more commonly, chemically as in Mayer’s Hematoxylin or Harris’s Hematoxylin solutions.
Commonly used in conjunction with eosin, hematoxylin solutions stain cell nuclei blue-black and provide exceptional intranuclear detail. Eosin Y staining will further result in pink, orange, or red cell cytoplasm and connective tissue fibers.
Rowley Biochemical offers a wide range of alum hematoxylin solutions for various applications.
Item numbers indicate where the product is applied to specific staining methods, but products are also available individually as “Special Order” (SO) items.
MAYER’S HEMATOXYLIN SOLUTIONS
Mayer’s Hematoxylin and Modified Mayer’s Hematoxylin are alum hematoxylins for routine staining with aluminum mordant.
- B-173, 3-Amino-9-Ethylcarbazole Method for Leukocyte Peroxidase
- D-252, Maximow’s Method for Bone Marrow
- E-303, Sweat-Puchtler Sirius Red Method
- E-305, Vassar-Culling Thioflavine T Method for Amyloid
- E-306, Laqueur’s Method for Alcoholic Hyalin
- E-310, Best’s Method for Glyocgen
- F-380, Fraser-Lendrum Method for Fibrin
- F-381, Carstair’s Method for Fibrin and Platelets
- F-401, Dane’s Method for Prekeratin, Keratin, and Mucin
- J-602, Rhodanine for Copper
- Special Order item SO-369
Special Order Item Mayer’s Hematoxylin SO-369.
Available in 4 oz, 8 oz, pint, quart, and gallon sizes.
MODIFIED MAYER’S HEMATOXYLIN SOLUTIONS
HARRIS’S HEMATOXYLIN SOLUTIONS
Harris’s Hematoxylin originally required toxic mercuric oxide for ripening, but modern solutions do not contain mercury. Harris’s Hematoxylin can be used as either a progressive or regressive stain providing especially clear staining of nuclei.
Item numbers indicate where the product is applied to specific staining methods, but products are also available individually as “Special Order” (SO) items.
- A-108, Taylor’s Method for Gram Positive and Gram Negative Bacteria
- A-109, Gridley’s Method for Endamoeba Histolytica
- A-123, Combined Hematoxylin and Eosin/Methenamine Silver Stain for Diagnosis of Fungi in Tissue Sections
- A-142, Page-Green Method for Inclusion Bodies
- E-312, McManus’ Method (PAS) for Glycogen
- F-390, Bielschowsky’s Method
- F-392, Wilder Modifiction of Bielschowsky’s Method
- F-396, Jones Method for Kidney
- H-500, Herxheimer’s Technique for Fat and Cholesterol
- H-504, Oil Red O in Propylene Glycol Method
- L-754, Harris’ Hematoxylin for Nuclei
- Special Order item SO-942
EHRLICH’S, DELAFIELD’S, AND GILL’S HEMATOXYLIN
Item numbers indicate where the product is applied to specific staining methods, but products are also available individually as “Special Order” (SO) items.
Other less-common alum hematoxylin solutions include:
- Ehrlich’s Hematoxylin
- H-503, Fat Stains in Supersaturated Isopropanol
- L-753, Ehrlich’s Hematoxylin for Nuclei
- Special Order item SO-325
Delafield’s Hematoxylin
Gill’s Hematoxylin (Types #1, #2, and #3)
- J-602, Rhodanine for Copper (Gill’s #2)
- L-759, Gill’s Hematoxylin for Histology (Gill’s #1, #2, and #3)
- M-801, Papanicolaou Stain for Membrane Filters (Gill’s #1)
- Special Order items SO-339 (Gill’s #1), SO-340 (Gill’s #2), SO-341 (Gill’s #3)
HEMALUM SOLUTIONS
Item numbers indicate where the product is applied to specific staining methods, but products are also available individually as “Special Order” (SO) items.
Mayer’s Acid Hemalum
- E-300, Benhold’s Congo Red for Amyloid
- E-311, Bauer’s Method for Glycogen
- L-756, Mayer’s Hematoxylin for Nuclei
- N-853, Periodic Acid Leucofuchsin (PAS) Method
- Special Order item SO-368
Alum Hematoxylin
Alum hematoxylins tend to be sensitive to acidic environments, limiting their use with other staining solutions such as van Gieson or trichrome stains. When acidic staining solutions are needed, iron-mordant hematoxylin solutions are required. The most common type is Weigert’s Iron Hematoxylin. In these methods, the co-staining solutions are Weigert’s Iron Hematoxylin Solution A and B.
Item numbers indicate where the product is applied to specific staining methods, but products are also available individually as “Special Order” (SO) items.
IRON HEMATOXYLIN SOLUTIONS
- A-102, Truant’s Fluorescent Method for Acid-Fast Bacteria
- A-109, Gridley’s Method for Endamoeba Histolytica
- E-320, Mucicarmine with Hematoxylin and Metanil Yellow
- F-350, Van Gieson’s Method for Collagen Fibers
- F-352, Biebrich Scarlet/Picric Aniline Blue Stain
- F-355, Lillie’s Allochrome Method
- F-362, Masson’s Trichrome Stain
- F-364, Lillie Modification of Masson’s Trichrome
- F-367, Masson’s Trichrome for Connective Tissue
- F-370, Weigert’s Resorcin Fuchsin
- F-374, Verhoeff’s van Gieson’s (VVG)
- F-375, Mollier’s Quadruple Stain
- F-383, Gomori’s One-Step Trichrome
- F-384, Movat’s Pentachrome Method
- F-386, Goldner’s Trichrome Method
- F-390, Bielschowsky’s Method
- G-470, Luna’s Method for Mast Cells
- G-481, Methenamine Silver Method for Argentaffin Cells
- G-486, Luna’s Method for Erythrocytes and Eosinophil Granules
- L-757, Weigert’s Iron Hematoxylin
- L-758, Weigert’s Iron Hematoxylin with Metachromic Dyes
- N-853, Periodic Acid Leucofucsin (PAS) Method
- Special Order items SO-465, SO-466
 Special Order Item Weigert’s Iron Hematoxylin A, SO-465. |
 Special Order Item Weigert’s Iron Hematoxylin B, SO-466. |
Heidenhain’s Hematoxylin Solution
PHOSPHOTUNGSTIC ACID (PTAH) HEMATOXYLIN SOLUTION
Tungsten Hematoxylin Solutions rely on the Mallory phosphotungstic acid (PTAH) method with tungsten acting as the mordant.
Item numbers indicate where the product is applied to specific staining methods.
- F-382, Mallory’s Phosphotungstic Acid Hematoxylin Method (PTAH)
ALCOHOLIC HEMATOXYLIN
Alcoholic Hematoxylins are also commonly sold as solutions in denatured alcohol. Item numbers indicate where the product is applied to specific staining methods, but products are also available individually as “Special Order” (SO) items.