NUCLEAR FAST RED

Nuclear Fast Red dye solutions (also known as Kernechtrot solutions) are useful for analyzing multiple sample types including hematologic elements, bone and calcium, carbohydrates and mucosubstances, connective tissue, and fats and lipids.  Often used in conjunction with silver oxidizing solutions.

NUCLEAR FAST RED FOR BLOOD AND HEMATOLOGIC ELEMENTS

Expected stain selectivity includes differentiation of blood corpuscles or leucocytes, Rickettsia, bacteria, inclusion bodies, malarial parasites, bacteria, Heinz bodies, reticulocytes, hemoglobin, and nuclei.  While no fixatives are often required, buffered neutral formalin (p/n SO-334) is the most common fixative for blood stains. Zenker’s (SO-687) or methanol (SO-372) are other choices.

Item numbers indicate where the product is applied to specific staining methods.

  • B-169, Turnbull Blue Method for Hemosiderin
  • Individual Special Order Item SO-130, Nuclear Fast Red Solution
Special Order Item Nuclear Fast Red SO-130. Available in 4 oz, 8 oz, pint, quart, and gallon sizes.

Special Order Item Nuclear Fast Red SO-130.
Available in 4 oz, 8 oz, pint, quart, and gallon sizes.

NUCLEAR FAST RED FOR BONE AND CALCIUM

Stains for bones and calcium are generally red, for example Nuclear Fast Red and Alizarin Red S (SO-636). Mayer’s Hematoxylin (SO-369) is also used. These stains are selective toward bones, teeth, and various calcium deposits and compounds. Alcohol and formalin are most commonly chosen for fixatives; in some cases decalcification fluids are recommended.

Item numbers indicate where the product is applied to specific staining methods.

  • C-211, Kossa’s Method for Calcium
  • C-212, Pizzolato’s Method for Calcium Oxalate

NUCLEAR FAST RED FOR MUCOSUBSTANCES

Selectivity reveals morphological details such as nuclei.  Specific methods for both weakly and strongly acidic sulfated mucosubstances are available.

Item numbers indicate where the product is applied to specific staining methods.

  • E-323, Alcian Blue Method (pH 2.5)
  • E-325, Alcian Blue Method (pH 0.4)

NUCLEAR FAST RED FOR CONNECTIVE TISSUE AND SILVER METHODS

For staining of basement membranes and silver methods for collagen and reticulum fibers in the central nervous system

Item numbers indicate where the product is applied to specific staining methods.

  • F-356, Puchtler-Sweat Method for Basement Membranes
  • F-392, Wilder Modification of Bielschowsky’s Method
  • F-394, Manuel’s Method for Reticulum
  • F-395, Snook’s Method for Reticulum

GRANULE STAINING WITH NUCLEAR FAST RED

Used in conjunction with silver reducing substances, nuclear fast red can aid in the detection of melanin and argentaffin granules.  Typically used in conjunction with Fontana’s Silver Nitrate (SO-605) or Ammoniacal Silver Solution (SO-134).

Item numbers indicate where the product is applied to specific staining methods.

  • G-484, Fontana-Masson Silver Method

NUCLEAR FAST RED FOR FATS AND LIPIDS

Item numbers indicate where the product is applied to specific staining methods.

Nuclear fast red helps discern fat in animal tissue.

  • H-502, Sudan Black B Stain

MINERAL AND PIGMENT STAINS WITH NUCLEAR FAST RED

Especially suitable in protocols for detection for minerals such as iron or mercury, nuclear fast red solutions are typically paired with potassium ferrocyanide (2% solution SO-406; 4% SO-730; 10% SO-408).  These systems will detect iron pigments as well as copper, nickel, and uranium in larger quantities.  Mercury is another common mineral target.

Item numbers indicate where the product is applied to specific staining methods.

  • J-601, Gomori’s Method for Iron
  • J-605, Mallory’s Method for Iron
  • J-606, Perl’s Method for Iron
  • J-615, Silver Method for Mercury